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Sep 12, 2021 · Vectors have been developed and adapted for a wide range of uses. Two primary uses are: (1) to isolate, identify, and archive fragments of a larger genome. (2) to selectively express proteins encoded by specific genes. Vectors were the first DNA tools used in genetic engineering, and continue to be cornerstones of the technology. Read more...
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DNA cloning is the process of making multiple, identical copies of a particular piece of DNA. In a typical DNA cloning procedure, the gene or other DNA fragment of interest (perhaps a gene for a medically important human protein) is first inserted into a circular piece of DNA called a plasmid.The insertion is done using enzymes that “cut and paste” DNA, and it Read more...
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DNA cloning can be used to make proteins such as insulin with biomedical techniques. It is used to develop recombinant versions of the non-functional gene to understand the functioning of the normal gene. This is applied in gene therapies also. It helps to analyse the effect of mutation on a particular gene. Also Read: Cloning Vectors Read more...
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Jul 20, 2018 · Human Cloning Pros and Cons. The pros or advantages of human cloning include: Infertility: Infertile people or same-sex couples could have children made from cloned cells. Organ replacement: A clone, like in the movie, "The Island," could be a source for transplant organs or tissue. (There are ethical issues that arise from this, however.) Read more...
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1 Theory. Molecular cloning is an essential technique to create DNA-based experimental tools for expression in bacterial or mammalian cells. Examples of such DNA constructs include a promoter element fused to a reporter gene or a cDNA sequence under the control of a ubiquitous promoter. Molecular cloning entails the preparation of the vector Read more...
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Jul 20, 2015 · Cloning genes or, more generally, cloning DNA segments is routinely done in many genetics and pharmaceutical laboratories throughout the world (12, 31). Technologies for cloning cells in the laboratory are seven decades old and are used for reproducing a particular type of cell, for example a skin or a liver cell, in order to Read more...
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Cloning humans? Biological, ethical, and social considerations
Nov 11, 2019 · Introduction. Human cloning is the creation of identical human being in the laboratory. It is an artificial process and is conducted with stem cell research and biotechnology. Reproductive cloning would give rise to human clones. Animal cloning is practiced from past few years and with the birth of Dolly, the sheep cloning has become Read more...
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